Tissue engineering of the esophagus has been limited by stent dependance and poor muscle regeneration. Here we report an integrated strategy to engineer a 2.5-cm esophageal segment by microinjecting autologous pericyte-like myogenic precursors and fibroblasts in a decellularized porcine scaffold to repair circumferential defects in 10-kg minipigs (n = 8), modeling pediatric use.
Natalie Durkin, George T. Hall, Roberto Lutman, Marianna Scuglia, Theodoros Xenakis, Giulia Patera, Daniele Di Biagio, Koji Yamada, Lucinda Tullie, Dominic Scaglioni, Soichi Shibuya, Kornilia Nikaki, Max Arran Beesley, Tarek Saleh, Matias Garrido Flores, Dominika Borselle, Valerija Karaluka, J. Ciaran Hutchinson, Sahira Khalaf, Olumide Ogunbiyi, Lei Wu, Xia Huang, Wenhui Song, Stavros Loukogeorgakis, Alessandro Filippo Pellegata, Sara Mantero, Giulio Cossu, Vivian S. W. Li, Osvaldo Borrelli, Paola Bonfanti, Sergi Castellano, Mattia Francesco Maria Gerli, Conor J. McCann, Simon Eaton, Marco Pellegrini & Paolo De Coppi
